human tissuescan quantitative pcr qpcr prostate cancer array iii  (OriGene)

Journal: Heliyon

Article Title: Novel roles for HMGA2 isoforms in regulating oxidative stress and sensitizing to RSL3-Induced ferroptosis in prostate cancer cells

doi: 10.1016/j.heliyon.2023.e14810

Figure Lengend Snippet: HMGA2-WT and -TR Expression in PCa and Breast cancer Cell Lines and Patients Tissue (A) Western blot analysis shows the expression of HMGA2 protein in prostate cancer and breast cancer cell lines, HMGA2-WT displays a higher molecular weight at about 23 KDa while the HMGA2-TR displays a lower molecular of about 17 KDa. (B) Western blot analysis measured the protein levels of both HMGA2-WT and -TR in prostate cancer patient tissue displaying different Stage/Gleason scores. (C) RT-PCR measured the mRNA levels in cDNA array. (D) Immunohistochemical analysis was performed using prostate tissue microarray with HMGA2 antibody in normal prostate tissue, hyperplasia, and chronic inflammation prostate tissue. Scale bar; 100 μm. Magnification 20X, inset 40X. Statistical analysis was conducted utilizing GraphPad Prism; (**** p < 0.0001, ***p < 0.001, **p < 0.01). Bars represents SD of the mean. Alpha-tubulin was used as a loading control. Results are representative of 3 independent experiments.

Article Snippet: Prostate normal and cancer tissue cDNA array (TissueScan, Prostate Cancer cDNA Array III- CAT#: HPRT103) was purchased from OriGene (Rockville, MD).

Techniques: Expressing, Western Blot, Molecular Weight, Reverse Transcription Polymerase Chain Reaction, Immunohistochemical staining, Microarray, Control

Journal: Future Oncology

Article Title: Role of opiorphin genes in prostate cancer growth and progression

doi: 10.2217/fon-2020-1299

Figure Lengend Snippet: The publicly available datasets GSE55945 and GSE3325 were analyzed using the GEO2R online tool to compare expression of opiorphin-encoding genes ( PROL1, SMR3A and SMR3B ) between cancerous and noncancerous prostate tissue samples.

Article Snippet: In order to confirm the upregulation of PROL1 in PrCa, a cDNA array from normal and cancerous prostate tissue was purchased from OriGene (HPRT103; TissueScanTM cDNA Arrays; Origene, MD, USA).

Techniques: Expressing

Journal: Future Oncology

Article Title: Role of opiorphin genes in prostate cancer growth and progression

doi: 10.2217/fon-2020-1299

Figure Lengend Snippet: (A) Quantitative real-time PCR was used to determine relative expression levels of PROL1 in a tissue cDNA microarray with samples from control tissue and different stages of PrCa. (B) The data for individual samples were normalized to the sample with the lowest level of PROL1 expression in the tissue array. Columns represent the average PROL1 expression level for each grade of cancer. There was significantly higher expression of PROL1 in PrCa tissue, with a trend for higher levels of expression as the cancer stage increased. *p < 0.05; error bars = standard deviation. PrCa: Prostate cancer.

Article Snippet: In order to confirm the upregulation of PROL1 in PrCa, a cDNA array from normal and cancerous prostate tissue was purchased from OriGene (HPRT103; TissueScanTM cDNA Arrays; Origene, MD, USA).

Techniques: Real-time Polymerase Chain Reaction, Expressing, Microarray, Control, Standard Deviation

Journal: Future Oncology

Article Title: Role of opiorphin genes in prostate cancer growth and progression

doi: 10.2217/fon-2020-1299

Figure Lengend Snippet: At 2 and 4 weeks post-xenograft, animals were euthanized and tumors excised, and the tumor tissue was used to isolate cells for cell culture. RNA was extracted from cells and tissues for RT-PCR for the PROL1 and VEGFA genes, with expression normalized to the housekeeping gene RPL19 . The relative change in expression of (A) PROL1 and (B) VEGFA is represented as fold change compared with the starting population of the parent PC3 cell line. *p < 0.05; n = 5; error bars = 95% CI.

Article Snippet: In order to confirm the upregulation of PROL1 in PrCa, a cDNA array from normal and cancerous prostate tissue was purchased from OriGene (HPRT103; TissueScanTM cDNA Arrays; Origene, MD, USA).

Techniques: Cell Culture, Reverse Transcription Polymerase Chain Reaction, Expressing

Journal: Future Oncology

Article Title: Role of opiorphin genes in prostate cancer growth and progression

doi: 10.2217/fon-2020-1299

Figure Lengend Snippet: PC3 and PC3-ProL1+ cell lines were injected into male and female nude mice and tumor size determined by standard caliper measurements (n = 10 per group). All mice developed tumors. The average tumor volume is shown for (A) male mice at early time points, (B) all time points for male mice and (C) all time points for female mice. *p < 0.05; error bars = standard error.

Article Snippet: In order to confirm the upregulation of PROL1 in PrCa, a cDNA array from normal and cancerous prostate tissue was purchased from OriGene (HPRT103; TissueScanTM cDNA Arrays; Origene, MD, USA).

Techniques: Injection

Journal: Future Oncology

Article Title: Role of opiorphin genes in prostate cancer growth and progression

doi: 10.2217/fon-2020-1299

Figure Lengend Snippet: Ontological groups containing overrepresented differentially expressed genes when PROL1 is overexpressed in PC3 cells.

Article Snippet: In order to confirm the upregulation of PROL1 in PrCa, a cDNA array from normal and cancerous prostate tissue was purchased from OriGene (HPRT103; TissueScanTM cDNA Arrays; Origene, MD, USA).

Techniques: Over Expression

Journal: Future Oncology

Article Title: Role of opiorphin genes in prostate cancer growth and progression

doi: 10.2217/fon-2020-1299

Figure Lengend Snippet: LNCaP and LNCaP-ProL1+ cell lines were injected into male, castrated male and female nude mice and tumor size determined by standard caliper measurements. The average tumor volume is shown for each time point. (A) Growth of tumors derived from LNCaP or LNCaP-ProL1 cell lines in male mice (n = 10 per group). All mice developed tumors. (B) Growth of tumors derived from LNCaP or LNCaP-ProL1+ cell lines in castrated male mice (n = 5 per group). Two of five mice developed tumors in the LNCaP-ProL1+ group and none of five developed tumors in the LNCaP group. (C) Growth of tumors derived from LNCaP or LNCaP-ProL1+ cell lines in female mice (n = 10 per group). All mice developed tumors. *p < 0.1; **p < 0.05; error bars = standard error.

Article Snippet: In order to confirm the upregulation of PROL1 in PrCa, a cDNA array from normal and cancerous prostate tissue was purchased from OriGene (HPRT103; TissueScanTM cDNA Arrays; Origene, MD, USA).

Techniques: Injection, Derivative Assay

Journal: Future Oncology

Article Title: Role of opiorphin genes in prostate cancer growth and progression

doi: 10.2217/fon-2020-1299

Figure Lengend Snippet: Ontological groups containing overrepresented differentially expressed genes when PROL1 is overexpressed in LNCaP cells.

Article Snippet: In order to confirm the upregulation of PROL1 in PrCa, a cDNA array from normal and cancerous prostate tissue was purchased from OriGene (HPRT103; TissueScanTM cDNA Arrays; Origene, MD, USA).

Techniques: Over Expression